indica, Oryza brachyantha, Oryza glaberrima and Oryza sativa subsp. The maximum number (twelve) of DJ-1 proteins were found to be present in Oryza sativa subsp.
The number of DJ-1/Pfp I domain containing proteins represented in Table 1 for various species may not represent the actual number in case of unsequenced genomes.
The maximum likelihood (ML) tree plotted using all the 217 sequences showed four major clades.
Out of 217 DJ-1 protein members, one hundred and seven proteins were found to be present in dicots (Table 1).
Apart from the model plant Arabidopsis thaliana, other species of Arabidopsis such as Arabidopsis lyrata and Arabidopsis halleri were also considered in the analysis. ten members of DJ-1 proteins were observed in Arabidopsis thaliana and Glycine max.
Site directed mutagenesis of Cys-119 to Alanine significantly reduces its GLY III activity indicating towards the existence of functional GLY III enzyme in rice—a shorter route for MG detoxification..
The pathway essentially consists of two enzymes, glyoxalase I (GLY I) and glyoxalase II (GLY II).
indica and Oryza brachyantha have five, Oryza minuta has two and minimum member present in Oryza punctata, Oryza officinalis and Oryza rufipogon.
Two Triticum species; Triticum urartu and Triticum aestivum were found to have three and thirteen members of the DJ-1 proteins, respectively. twenty-one DJ-1 proteins were observed in Hordeum vulgare var. Among others, Zea mays has eleven, Setaria italica and Sorghum bicolor have five, Musa acuminata has four, Brachypodium distachyon has three and Aegilops tauschii has only one DJ-1/Pfp I domain containing proteins (Table 1).
A member of Os DJ-1 family, Os DJ-1C, showed high constitutive expression at all developmental stages and tissues of rice.